This Circular stipulates economic and technical norms for implementing DNA identification services to determine the identity of martyrs' remains with missing information, applicable to agencies and organizations commissioned by the Ministry of Labor, Invalids and Social Affairs. It provides detailed regulations on tasks, machinery and equipment, and the number of laborers required to perform DNA identification.
적용 범위
Agencies and organizations commissioned by the Ministry of Labor, Invalids and Social Affairs to implement DNA identification services using samples of martyrs' remains and relatives' samples to determine the identity of martyrs' remains with missing information.
핵심 사항
- Agencies and organizations commissioned by the Ministry of Labor, Invalids and Social Affairs to implement DNA identification services using samples of martyrs' remains and relatives' samples to determine the identity of martyrs' remains with missing information.
- Economic and technical norms for analyzing samples of martyrs' remains, extracting DNA, PCR Amplify, electrophoresis, purifying cycle sequencing reaction products, sequencing, sequence analysis, and issuing identification results.
- Work related to the preservation of samples of martyrs' remains and relatives' comparison samples.
- Economic and technical norms for analyzing relatives' comparison samples, extracting DNA, PCR Amplify, electrophoresis, purifying cycle sequencing reaction products, sequencing, sequence analysis, and issuing identification results.
- Necessary work such as sample processing before storage, chemical usage, and labor protection equipment.
🌐 이 문서의 사회적 영향
- To accurately and quickly identify the identity of martyrs' remains with missing information.
- Save time and costs for the DNA identification process.
- Enhance the preservation work of samples of martyrs' remains and relatives' comparison samples.
❓ 자주 묻는 질문
Which agencies and organizations can carry out DNA identification services?
Agencies and organizations commissioned by the Ministry of Labor, Invalids and Social Affairs to carry out DNA identification services using samples of martyrs' remains and relatives' samples to determine the identity of martyrs' remains with missing information.
What processes are included in the economic and technical norms for analyzing samples of martyrs' remains?
The economic and technical norms for analyzing samples of martyrs' remains include processes such as sample preservation, DNA extraction, PCR Amplify, electrophoresis, purification of cycle sequencing reaction products, sequencing, sequence analysis, and issuing identification results.
Which task requires the most labor?
The task of DNA extraction (QT03.01/HCLS) and PCR Amplify (QT03.04/HCLS) requires the most labor, each process requiring four people.
What processes are included in the economic and technical norms for analyzing relatives' comparison samples?
The economic and technical norms for analyzing relatives' comparison samples include processes such as sample preservation, DNA extraction, PCR Amplify, electrophoresis, purification of cycle sequencing reaction products, sequencing, sequence analysis, and issuing identification results.
Which task requires the most chemicals?
The task of DNA extraction (QT03.01/HCLS) and PCR Amplify (QT03.04/HCLS) requires the most chemicals, each process using 40 mL of absolute ethanol.
전문
CIRCULAR
ISSUING REGULATIONS ON ECONOMIC AND TECHNICAL STANDARDS FOR DNA FORENSIC SERVICES TO IDENTIFY THE IDENTITY OF REMAINS OF UNKNOWN SOLDIERS
THE MINISTER OF LABOR, WAR INVALIDS AND SOCIAL AFFAIRS
Pursuant to Decree No.Decision No. 62/2022/NĐ-CP September 12, 2022 of the Government on the functions, tasks, powers, and organizational structure of the Ministry of Labor - Invalids and Social Affairs;
Pursuant to Decree No.131/2021/NĐ-CP December 30, 2021 Government Decree detailing and providing implementation measures for the Ordinance on Preferential Treatment for Persons Contributing to the Revolution;
Pursuant to Decree No.55/2023/NĐ-CP July 21, 2023 Government Decree amending and supplementing certain articles of the Government Decree number75/2021/NĐ-CP July 24, 2021 Government Decree stipulating the level of allowances, subsidies, and preferential policies for persons contributing to the revolution;
Pursuant to Decree No.60/2021/NĐ-CP June 21, 2021 Government Decree stipulating the financial autonomy mechanism for public service units;
Pursuant to Decree No.73/2024/NĐ-CP June 30, 2024 Government Decree stipulating the base salary and bonus system for civil servants, public officials, and military personnel;
Pursuant to Circular No. 23/2023/TT-BTC April 25, 2023 of the Ministry of Finance guiding the management, depreciation, and amortization of fixed assets at agencies, organizations, units, and fixed assets entrusted by the State to enterprises without being considered part of the State's capital in the enterprise;
Pursuant to Circular No. 119/2023/TT-BQP December 22, 2023 of the Ministry of National Defense promulgating the procedure for identifying the identity of remains of unknown soldiers;
Pursuant to Decision No. 1771/QĐ-LĐTBXH dated December 26, 2016 of the Minister of Labor, Invalids and Social Affairs regarding the issuance of the Regulation on the management of economic and technical standards under the jurisdiction of the Ministry of Labor, Invalids and Social Affairs;
At the proposal of the Director of the Department of Persons Contributing to the Revolution, the Minister of Labor, Invalids and Social Affairs issues this Circular on economic and technical standards for implementing DNA forensic services to identify the identity of remains of unknown soldiers.
Article 1. Scope of Regulation
This Circular stipulates economic and technical standards for implementing DNA forensic services to identify the identity of remains of unknown soldiers.
Article 2. Applicability
This Circular applies to agencies and organizations commissioned by the Ministry of Labor, Invalids and Social Affairs to perform DNA forensic services on samples of remains of unknown soldiers and samples of relatives of soldiers to determine the identity of remains of unknown soldiers.
Article 3. Explanation of Terms
1. DNA forensic service is a public service using state budget funds within the social security sector.
2. Economic and technical standards for DNA forensic services to identify the identity of remains of unknown soldiers are the amount of consumption of labor factors, equipment, and materials required to complete the analysis of one DNA sample (one sample of remains of an unknown soldier or one sample of a relative of a soldier).
Article 4. Content of Standards
The content of economic and technical standards for DNA forensic services to identify the identity of remains of unknown soldiers includes economic and technical standards for mitochondrial DNA analysis of samples of remains of unknown soldiers and economic and technical standards for mitochondrial DNA analysis of samples of relatives of soldiers (two Appendices attached).
Article 5. Implementation Provisions
This Circular takes effect from April 13, 2025.
Article 6. Implementation Organization
During the implementation process, if there are any difficulties, they should be reported promptly to the Ministry of Labor, Invalids and Social Affairs (through the Department of Persons Contributing to the Revolution) for consideration and resolution within their authority or reporting to the competent authority for resolution.
VICE MINISTER
ANNEX I
ECONOMIC AND TECHNICAL STANDARDS FOR MITOCHONDRIAL DNA ANALYSIS OF SAMPLES OF REMAINS OF UNKNOWN SOLDIERS
(Attached to Circular No. 05/2025/TT-BLDTBXH dated February 26, 2025 of the Minister of Labor, Invalids and Social Affairs)
|
No. |
Content of work |
Quantity and unit of measurement |
Necessary machinery and equipment |
Labor hours (excluding machine operation time) |
Remarks |
|
I |
Sample analysis of remains of unknown soldiers |
||||
|
1 |
Storage of samples of remains of unknown soldiers |
||||
|
1.1 |
Pre-treatment before storage |
2 hours/person |
Number of people 02 (01 analyst + 01 technician) |
||
|
Cleaning paper |
|||||
|
Surface cleaning chemicals |
|||||
|
Sample container |
|||||
|
Underwear |
02 pairs |
||||
|
Face mask |
02 pieces |
||||
|
Protective glasses |
02 pieces |
||||
|
Chemical-resistant protective clothing |
02 sets |
||||
|
Scalpel |
01 piece |
||||
|
1.2 |
Immediate-use sample stored at 4°C to 10°C |
Refrigerator |
|||
|
1.3 |
Long-term storage sample at -20°C |
Refrigerator -20°C to -30°C |
|||
|
2 |
DNA extraction (QT03.01/HCLS) |
8 hours/person |
Number of people 04 (02 analysts + 02 technicians) |
||
|
Cleaning paper |
Similar chemicals and equipment may be used |
||||
|
Surface cleaning chemicals |
|||||
|
Sample container |
|||||
|
2.1 |
Sample processing |
||||
|
Absolute ethanol |
40mL |
Ultrasonic bath |
|||
|
UltraPure™ DNase/RNase-Free Distilled Water |
40mL |
Dremel tool |
|||
|
Sodium hypochlorite |
40mL |
Bone grinder |
Surface cleaning, grinding bone into powder. |
||
|
15mL centrifuge tube |
07 pieces |
||||
|
Underwear |
04 pairs |
||||
|
Face mask |
04 pieces |
||||
|
Protective glasses |
04 pieces |
||||
|
Chemical-resistant protective clothing |
04 sets |
||||
|
Scalpel |
01 piece |
||||
|
2.2 |
De-mineralization |
||||
|
|
UltraPure™ 0.5M EDTA, pH 8.0 |
12.4mL |
Vortex mixer |
||
|
Proteinase K 20mg/mL |
300µL |
||||
|
UltraPure™ DNase/RNase-Free Distilled Water |
1.5mL |
Removal of minerals, calcium, and organic matter |
|||
|
Tris HCl pH7.5 1M |
400 µL |
||||
|
Sodium hypochlorite |
10mL |
||||
|
Absolute ethanol |
02mL |
||||
|
Underwear |
04 pairs |
||||
|
Face mask |
04 pieces |
||||
|
Protective glasses |
04 pieces |
||||
|
Chemical-resistant protective clothing |
01 set |
||||
|
15mL centrifuge tube |
02 pieces |
||||
|
1000µL pipette tips |
04 pieces |
||||
|
200µL pipette tips |
10 pieces |
||||
|
2.0mL Eppendorf tube |
04 pieces |
||||
|
2.3 |
Removal of proteins and impurities |
||||
|
Phenol:Chloroform:Isopropyl Alcohol (25:24:1) |
03 mL |
Centrifuge machine |
Extracting DNA from bone powder samples |
||
|
Chloroform: Isoamyl Alcohol (24:1) |
03mL |
Thermal shaker |
|||
|
Eppendorf tube 2mL |
10 pieces |
Vortex mixer |
|||
|
1000µL pipette tips |
15 units |
Refrigerator at 4°C |
|||
|
Underwear |
04 pairs |
||||
|
Face mask |
04 pieces |
||||
|
2.4 |
Ethanol precipitation |
||||
|
Absolute ethanol |
7.5mL |
Refrigerator at 4°C |
|||
|
|
Sodium acetate solution 3M |
200µl |
Refrigerator at -80°C |
||
|
1000µL pipette tips |
03 units |
Refrigerator at -20°C |
|||
|
200µL pipette tips |
03 units |
||||
|
Underwear |
04 pairs |
||||
|
Face mask |
04 pieces |
||||
|
Fancol tube 50mL |
01 piece |
||||
|
Eppendorf tube 2mL |
01 piece |
||||
|
2.5 |
Purify extracted DNA |
||||
|
Absolute ethanol |
500µl |
Refrigerator at -80°C |
|||
|
Wizard® SV Gel and PCR Clean-up System |
01 sample |
Refrigerator at -20°C |
|||
|
UltraPure™ DNase/RNase-Free Distilled Water |
200µl |
||||
|
Eppendorf tube 1.5mL |
04 pieces |
||||
|
1000µL pipette tips |
02 pieces |
||||
|
200µL pipette tips |
14 units |
||||
|
3 |
PCR AmplificationpNumber of employees and workers directly involved in the joint venture and association activities of the organization over the last 03 yearsfy (QT03.04/HCLS) |
04 hours/1 person |
Number of people 02 (01 forensic examiner + 01 technician) |
||
|
HotStar Taq |
16 reactions |
Class II Biological Safety Cabinet |
Amplifying target gene segments/number of primer pairs |
||
|
Primer (06 pairs) |
12 reactions |
PCR machine |
|||
|
MgCl2 |
120µl |
Centrifuge |
|||
|
Eppendorf tube 0.5mL |
20 units |
Vortex mixer |
|||
|
Eppendorf tube 0.2mL |
48 units |
Refrigerator at 4°C |
|||
|
|
1000µL pipette tips |
06 units |
Refrigerator at -80°C |
||
|
200µL pipette tips |
24 units |
Refrigerator at -20°C |
|||
|
Pipette tips 10 |
36 units |
||||
|
Underwear |
02 pairs |
||||
|
Face mask |
02 pieces |
||||
|
Protective glasses |
02 pieces |
||||
|
Chemical-resistant protective clothing |
02 sets |
||||
|
Cleaning paper |
|||||
|
Surface cleaning chemicals |
|||||
|
Sample container |
|||||
|
4 |
Electrophoresis (QT03.05/HCLS) |
0.5 hours/1 person |
Number of people 02 (01 forensic examiner+01 technician) |
||
|
RedSafe |
5µl |
Observe PCR reaction results on gel and purify gel if PCR reaction meets requirements |
|||
|
Agarose |
1g |
Electrophoresis tank |
|||
|
100bp DNA ladder |
10µL |
Gel imaging system |
|||
|
TBE 10X |
120ml |
Vortex mixer |
|||
|
DNA Loading Dye (6X) |
20µl |
Thermal shaker |
|||
|
200µL pipette tips |
6 units |
Analytical balance |
|||
|
Pipette tips 10 |
18 units |
Magnetic stirrer |
|||
|
Underwear |
02 pairs |
Refrigerator at 4°C |
|||
|
Protective glasses |
02 pieces |
Refrigerator at -20°C |
|||
|
|
Chemical-resistant protective clothing |
0.1 set |
Camera |
||
|
Face mask |
0.2 unit |
||||
|
Cleaning paper |
|||||
|
Surface cleaning chemicals |
|||||
|
Sample container |
|||||
|
5 |
Purification (QT03.06/HCLS) |
1 hour/1 person |
Number of people 02 (01 forensic examiner + 01 technician) |
||
|
X-tracta Gel Extraction Tool |
12 |
Eppendorf centrifuge |
|||
|
GeneJET Genomic DNA Purification Kit |
12 |
Vortex mixer |
|||
|
TBE 10X |
120ml |
Thermal shaker |
|||
|
DNA Loading Dye (6X) |
24µl |
Analytical balance |
|||
|
200µL pipette tips |
12 units |
||||
|
1000µL pipette tips |
12 units |
Refrigerator at 4°C |
|||
|
Underwear |
03 units |
Refrigerator at -20°C |
|||
|
Eppendorf tube 1.5mL |
12 units |
||||
|
15mL centrifuge tube |
01 piece |
||||
|
Face mask |
02 pieces |
||||
|
Cleaning paper |
|||||
|
Surface cleaning chemicals |
|||||
|
Sample container |
|||||
|
6 |
Cycle sequencing (QT.03.07/HCLS) |
1 hour/1 person |
Number of people 02 (01 forensic examiner + 01 technician) |
||
|
|
BigDye™ Terminator v3.1 Cycle Sequencing Kit |
12 reactions |
Refrigerator at 4°C |
||
|
Primer (12 single primers) |
12 reactions |
Refrigerator at -20°C |
|||
|
200µL pipette tips |
12 units |
||||
|
Pipette tips 10 |
24 units |
||||
|
Underwear |
02 pairs |
||||
|
Face mask |
02 pieces |
||||
|
7 |
Purify cycle sequencing reaction products and sequence (QT03.08/HCLS) |
1 hour/1 person |
Number of people 03 (02 forensic examiners + 01 technician) |
||
|
ZR DNA Sequencing Clean-up Kit™ (200 Preps) w/ Zymo Spin IB |
12 reactions |
ABI 3500 analyzer capillary electrophoresis machine |
Sequence amplified gene segments from above step |
||
|
2.0mL Eppendorf tube |
12 units |
PCR machine |
|||
|
200µL pipette tips |
24 units |
Analysis required |
|||
|
1000µL pipette tips |
12 units |
Magnetic stirrer |
|||
|
3500 Genetic Analyzer 8-capillary array, 50 cm |
12 reactions |
Vortex mixer |
|||
|
POP-7 Polymer for 3500/3500xL Genetic Analyzers |
12 reactions |
Centrifuge machine |
|||
|
Anode Buffer Container (ABC) 3500 Series, 4 packs/box |
12 reactions |
Refrigerator at 4°C |
|||
|
Cathode Buffer Container (CBC) 3500 Series, 4 packs/box |
12 reactions |
||||
|
|
MicroAmp Optical 96-Well Reaction Plate with Barcode |
12 wells |
|||
|
Underwear |
03 pairs |
||||
|
Face mask |
03 units |
||||
|
8 |
Sequence analysis (QT03.09/HCLS) |
2 hours/1 person |
Number of people 02 (01 forensic examiner + 01 technician) |
||
|
Computer analysis |
Analyze results and compare results |
||||
|
Server to store results |
|||||
|
Analysis software |
|||||
|
Analysis software |
|||||
|
9 |
Compile forensic examination results |
2 hours/1 person |
Number of forensic examiners 02 |
||
|
10 |
Store DNA and remains of烈士的遗体样本和DNA的保管l后鉴定（使用-20°C冰箱） |
||||
ANNEX II
鉴定烈士体细胞样本亲属鉴定经济和技术定额
（由劳动和社会保障部令第05/2025/TT-BLĐTBXH号于2025年2月26日发布）
|
No. |
Content of work |
Quantity and unit of measurement |
Necessary machinery and equipment |
劳动时间（小时）不包括机器运行时间 |
Remarks |
|
II |
分析烈士亲属样本 |
||||
|
1 |
保存烈士亲属样本 |
0.5小时/人 |
Number of people 02 (01 forensic examiner + 01 technician) |
||
|
Cleaning paper |
|||||
|
Surface cleaning chemicals |
|||||
|
Sample container |
|||||
|
Underwear |
02 pairs |
||||
|
1.1 |
即时使用的生物样本在4°C到10°C下保存 |
Refrigerator |
|||
|
1.2 |
长期保存的生物样本在-20°C下保存 |
Refrigerator -20°C to -30°C |
|||
|
2 |
提取DNA（QT03.02/HCLS） |
2小时/人 |
Number of people 04 (02 analysts + 02 technicians) |
||
|
PBS |
01mL |
||||
|
Chelex 100 |
0.01g |
||||
|
Proteinase K 20mg/mL |
10µL |
||||
|
1000µL pipette tips |
02 pieces |
||||
|
200µL pipette tips |
02 pieces |
||||
|
2.0mL Eppendorf tube |
02 pieces |
||||
|
Fancol tube 50mL |
04 pieces |
||||
|
Underwear |
04 pairs |
||||
|
Face mask |
04 pieces |
||||
|
Protective glasses |
04 pieces |
||||
|
Chemical-resistant protective clothing |
04 sets |
||||
|
3 |
PCR AmplificationpNumber of employees and workers directly involved in the joint venture and association activities of the organization over the last 03 yearsfy (QT03.04/HCLS) |
02小时/人 |
Number of people 02 (01 forensic examiner + 01 technician) |
||
|
HotStar Taq |
06反应 |
Class II Biological Safety Cabinet |
|||
|
引物（02对） |
06反应 |
PCR machine |
|||
|
Eppendorf tube 0.5mL |
12 units |
Vortex mixer |
扩增目标基因片段 |
||
|
Eppendorf tube 0.2mL |
12 units |
||||
|
200µL pipette tips |
06 units |
||||
|
Pipette tips 10 |
15 units |
||||
|
Underwear |
02 pairs |
||||
|
Face mask |
02 pieces |
||||
|
Protective glasses |
02 pieces |
||||
|
Chemical-resistant protective clothing |
02 sets |
||||
|
4 |
Electrophoresis (QT03.05/HCLS) |
0.5小时/人 |
Number of people 02 (01 forensic examiner + 01 technician) |
||
|
RedSafe |
2.5µl |
||||
|
|
Agarose |
0.5g |
电泳槽 |
||
|
100bp DNA梯 |
10µL |
Gel imaging system |
|||
|
TBE 10X |
120ml |
Vortex mixer |
Observe PCR reaction results on gel and purify gel if PCR reaction meets requirements |
||
|
DNA Loading Dye (6X) |
10µl |
Thermal shaker |
|||
|
200µL pipette tips |
2 pieces |
Analytical balance |
|||
|
Pipette tips 10 |
6 units |
Magnetic stirrer |
|||
|
Underwear |
01 piece |
Refrigerator at 4°C |
|||
|
Protective glasses |
01 piece |
Refrigerator at -20°C |
|||
|
Chemical-resistant protective clothing |
0.1 set |
||||
|
Face mask |
0.2 unit |
||||
|
5 |
Purification (QT03.06/HCLS) |
1 hour/1 person |
Number of people 02 (01 forensic examiner + 01 technician) |
||
|
X-tracta Gel Extraction Tool |
06 units |
Camera |
|||
|
GeneJET Genomic DNA Purification Kit |
06 units |
Eppendorf centrifuge |
|||
|
200µL pipette tips |
06 units |
Analytical balance |
|||
|
1000µL pipette tips |
06 units |
Magnetic stirrer |
|||
|
|
Underwear |
02 pairs |
Refrigerator at 4°C |
||
|
Eppendorf管1.5mL |
02 pieces |
Refrigerator at -20°C |
|||
|
Fancol管15mL |
01 piece |
||||
|
Face mask |
02 pieces |
||||
|
6 |
Cycle sequencing (QT.03.07/HCLS) |
1 hour/1 person |
Number of people 02 (01 forensic examiner + 01 technician) |
||
|
BigDye™ Terminator v3.1 Cycle Sequencing Kit |
06反应 |
Refrigerator at 4°C |
|||
|
引物（06对引物） |
06反应 |
Refrigerator at -20°C |
序列化上述步骤扩增的基因片段 |
||
|
200µL pipette tips |
10 pieces |
||||
|
Pipette tips 10 |
10 pieces |
||||
|
Underwear |
02 pairs |
||||
|
Face mask |
02 pieces |
||||
|
7 |
Purify cycle sequencing reaction products and sequence (QT03.08/HCLS) |
1 hour/1 person |
Number of people 04 (02 analysts + 02 technicians) |
||
|
ZR DNA Sequencing Clean-up Kit™ (200 Preps) w/ Zymo Spin IB |
06反应 |
Analytical balance |
|||
|
UltraPure™ DNase/RNase-Free Distilled Water |
100µl |
Magnetic stirrer |
|||
|
|
2.0mL Eppendorf tube |
06 units |
ABI 3500毛细管电泳仪 |
||
|
200µL pipette tips |
10 pieces |
||||
|
1000µL pipette tips |
10 pieces |
||||
|
3500 Genetic Analyzer 8-capillary array, 50 cm |
06反应 |
||||
|
POP-7 Polymer for 3500/3500xL Genetic Analyzers |
06反应 |
PCR machine |
|||
|
Anode Buffer Container (ABC) 3500 Series, 4 packs/box |
06反应 |
Vortex mixer |
|||
|
Cathode Buffer Container (CBC) 3500 Series, 4 packs/box |
06反应 |
Centrifuge machine |
|||
|
MicroAmp Optical 96-Well Reaction Plate with Barcode |
08井 |
||||
|
Underwear |
04 pairs |
||||
|
Face mask |
04 pieces |
||||
|
8 |
Sequence analysis (QT03.09/HCLS) |
2小时/人 |
人数03（02鉴定人员+01技术人员） |
||
|
Computer analysis |
Analyze results and compare results |
||||
|
Server to store results |
|||||
|
分析软件Sequencher |
|||||
|
Part of software for sequencing analysis |
|||||
|
9 |
Compile forensic examination results |
2小时/人 |
Number of forensic examiners 02 |
||
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